Investigation of the effects on surface plasmon resonance (SPR) in glycerol solution sensing

Nik Wan, Nik Mazni (2020) Investigation of the effects on surface plasmon resonance (SPR) in glycerol solution sensing. Project Report. Universiti Teknikal Malaysia Melaka, Melaka, Malaysia. (Submitted)

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Abstract

Surface plasmon resonance (SPR) is a process that occurs on metal surfaces usually gold and silver when the light of incident hit the surface at a specific angle. The SPR effect lead to significant decrease in the reflected light intensity. Biomedical applications benefit from the SPR's exquisite sensitivity to the medium refractive index next to the surface of metal, enable precise measurement of the adsorption of molecules on metal surfaces and its potential interactions with different ligands. The last ten years have seen huge developments in the use of SPR in applications of the biomedical. The technique is used not only to test the kinetics of interactions between ligand receptors in real-time and to screen lead compounds in the pharmaceutical industry, but also to test DNA hybridization, enzyme substrate interactions, tests on protein conformation, and label-free immunoassays. Due to the existing technology which is Glycerol Bio to test kit for Cedex Bio Analyzer is too expensive. So, this project proposes to develop an inexpensive optical sensor based on SPR concept to detect different concentrations of glycerol solution. This project successfully applied SPR to glycerol solution using colloidal gold particles. This technology provides many advantages over conventional SPR. By using gold, the Phenomenon of SPR can be analyzed in photodetector by using LCD to show the output voltage of the glycerol solution sensing.

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