Investigation on mechanical and microscopic characteristic of seaweed-based hydrogel for three-dimensional bioprinting

Kirno, Muhammad Azlan (2024) Investigation on mechanical and microscopic characteristic of seaweed-based hydrogel for three-dimensional bioprinting. Project Report. Universiti Teknikal Malaysia Melaka, Melaka, Malaysia. (Submitted)

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Abstract

The utilization of hydrogels derived from seaweed, characterized by gel-like properties, has gained prominence in bio-ink materials due to their ability to emulate the mechanical attributes of living tissues. There is tremendous potential of hydrogels generated from seaweed for bio-printing, offering a promising route for environmentally friendly and sustainable substitutes for traditional synthetic bio-ink materials. Nowadays, there is a significant lack of understanding about the best practices for preparing and working with these materials when it comes to bio-printing. The objective of this study is to develop a seaweed-based bio-ink for bio-printing, optimize the bio-printing parameters for the seaweed-based materials, and examine the mechanical and microstructural properties of the resulting seaweed-based prints. The mechanical characteristics of the hydrogel, including its strength and resistance to deformation, were evaluated by puncture tests. Furthermore, the microstructure and characteristic of the seaweed-based hydrogel were examined using (SEM), which provided a greater resolution of image analysis.it was found that the viscosity of the bioink increases with seaweed based bioink. The best seaweed-based hydrogel formulation for extrusion was 6.72dPas for 1% seaweed and 1% alginate. Strength of the seaweed based bioink increases, up to 1.60Mpa. Microstructural analysis of 1%nseaweed and 1% alginate exhibit larger size and amount of porosity which is necessary for cell culture in the scaffold. The results from the study may contribute to the advancement of new biomaterial variations that improve cell culture in medical treatments, especially in utilizing locally resourced materials.

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